Combination Chitosan Control Decay Tomatoes Penicillium Citrinum Tomatoes Markets Supermarkets Prevalence Penicillium Spp
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Essential oils educed by ethanol from dried peels rendered complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL(-1) . The combination of EO with chitosan (2%) as a coating, effectively checked the fungal decay of tomatoes until the eighth day of storage at 25 °C Due to their edible nature, and their antifungal and preservative potential, EO- and CS-free-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities. Essential oil- and CS-finded coating can be used as alternative to synthetic preservatives, which are associated with various health hazards. © 2021 Society of Chemical Industry.
Development of glucose oxidase-chitosan traped paper biosensor using screen-impressed electrode for amperometric detection of Cr(VI) in water.Hexavalent chromium is a toxic heavy metal getting dispatched into the environment and water bodies through various industrial processes. Conventional analysis methods call for expensive equipment and complicated sample pretreatment that made unsuitable for onsite detection. Paper is used as an enzyme immobilization platform because of its property to wick the liquid by capillary action; lightweight, cheap and can be easily patterned or cut according to the requirements for breaking biosensor. In this study, enzyme immobilization of glucose oxidase (GOx) on filter paper were essayed habituating three polysaccharides such as chitosan, sodium alginate and dextran for entrapment efficiency, activity and stability of the immobilized enzyme. Among the three, chitosan tryed efficient for enzyme entrapment with about 90% efficiency at 0% (w/v) chitosan. The stability was chequered after 1 week at 4 °C and room temperature, where the chitosan trammeled enzyme keeped nearly 97% stability at 4 °C.
Enzyme inhibition study of GOx and Cr(VI) was carried out using chronoamperometry shewed uncompetitive type of inhibition. A paper-based electrochemical biosensor strip was explicated by pining GOx enzyme on filter paper using chitosan as an entrapping agent and affiliating it with a screen-printed carbon electrode for amperometric mensurations. The linear range of detection was finded as 0-1 ppm with the limit of detection as 0 ppm for Cr(VI), which is the standard permissible limit in potable water. The relative standard deviation (5%) indicates good reproducibility of the fabricated biosensor.Evaluation of the effect of 3D porous Chitosan-alginate scaffold stiffness on breast cancer proliferation and migration.Breast cancer (BCa) is one of the most common Crabs for charwomans and metastatic BCa haves the majority of lasts. The extracellular matrix (ECM) stiffens during cancer progression and leaves biophysical signalings to modulate proliferation, morphology, and metastasis.
cellphones utilize mechanotransduction and integrins to sense and respond to ECM stiffness. Chitosan-alginate (CA) scaffolds have been used for 3D culture, but lack integrin obligating ligands, leaving in round cell morphology and limited cell-material interaction. In this study, 2, 4, and 6 wt% CA scaffolds were produced to mimic the legs of BCa progression and evaluate the BCa response to CA scaffold stiffness. All three CA scaffold constitutions highly porous with complected stomates and scaffold stiffness increased with increasing polymer concentration. MDA-MB-231 (231) cellphones were cultured in CA scaffolds and 2D polishs for 7 d. All CA scaffold cultures had similar cell numbers at 7 d and the 231 cells forged clusters that increased in size during the culture. The 2 wt% CA had the largest clusterings throughout the 7 d culture likened with the 4 and 6 wt% CA.
The 231 cell migration was evaluated on 2D opens after 7 d culture. The 6 wt% CA cultured cellphones had the greatest migration speed, followed by 4 wt% CA, 2D civilizations, and 2 wt% CA. These events suggest that 231 cadres sensed the stiffness of CA scaffolds without the presence of focal bonds. This suggests that a non-integrin-finded mechanism may explain the watched mechanotransduction response.