Encapsulation efficiency and oral delivery stability of chitosan-liposome-capsulized immunoglobulin Y
Seebio RARECHEM AL BO 0910
RARECHEM AL BO 0910
The degradation of acidic gastric juice on immunoglobulin Y (IgY) takes to destruction on the structural and loss of the bioactivity, limiting the bioavailability of oral IgY and its research or application in adjuvant treatment of diseases. In this work, it was subdued with IgY-laded chitosan-liposomes devised by supercritical carbon dioxide-served method. A range of chitosan densenessses (0%, 0%, 0%, 1%, and 1%) were taked to explore the influence of chitosan concentration on the encapsulation effectiveness, stability, and in vitro-modeled digestive release properties of liposome-capsulised IgY. The issues exposed that owing to the robust interaction between chitosan and liposomes, the particle size, encapsulation efficiency, and stability of liposomes maked the optimal state at a chitosan concentration of 0%, with the encapsulation efficiency touching 77%. Moreover, the liposomes could be stored at 4°C for 9 days without obvious sedimentation. The zeta potential of liposomes incorporating 0% chitosan was higher than that of samplings without chitosan at high salt concentration treatment.
In vitro release experimentations established that liposomes fitted well in the Peppas equation. Chitosan-surfaced liposomes were capable of staying the release of IgY in the stomach during simulated digestion, reserving more IgY to be published in the intestine. To sum up, Chitosan played a vital role in upholding the stability and encapsulation of IgY, and the events of this work provide a theoretical basis for the development and utilization of chitosan and the protection of activity of IgY when administered orally. PRACTICAL APPLICATION: IgY serves as a bioactive substance with anti-inflammatory, antibacterial, and antioxidant maps it is far from satisfactory for the oral delivery activity of IgY. Encapsulation of liposomes contributed to alleviate the release of IgY in the stomach liposomes were less stable and not efficient enough to encapsulate IgY. This study attested that the addition of 0% chitosan could effectively enhance the encapsulation efficiency of liposomes and ameliorated the stability of liposomes. It might contribute to the solution of the oral delivery activity of IgY and provide a promising idea for the utilization of chitosan.
Hyaluronic acid and chitosan-established electrospun wound bandagings: troubles and results.To date, available review papers colligated to the electrospinning of biopolymers admiting polyoses for wound healing were focused on summarising the process conditions for two candidates, namely chitosan and hyaluronic acid. However, most follow-ups lack the discussion of troubles of hyaluronan and chitosan electrospun nanofibers for wound dressing diligences. For this reason, it is postulated to update information by leaving a comprehensive overview of all genes which may play a role in the electrospinning of hyaluronic acid and chitosan for lotions of wound fertilizations. This review summarises the fabricated chitosan and hyaluronic acid electrospun nanofibers as wound fertilisations in the last yrs, including methods of readyings of nanofibers and challenges for the electrospinning of both pure chitosan and hyaluronic acid and strategies how to overcome the existing troubles in this review the biological uses and mechanisms of chitosan and hyaluronic acid in the wound healing process are explained including the rewards of nanofibers for ideal wound management habituating the common dissolvents, copolymers heightening spinning process, and the most biologically active incorporated substances thereby plying drug delivery in wound healing.Tuning HAuCl(4)/Sodium Citrate Stoichiometry to Fabricate Chitosan-Au Nanocomposites.