UV Cross-Linked Injectable Non-Swelling Dihydrocaffeic Acid Transplanted Chitosan Hydrogel For Advancing Wound Healing

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Hydrogels are widely used as wound fecundations for wound healing, but when hydrogels absorb wound exudate, tumescing haps and compacts the ringing tissue, affecting healing. A chitosan injectable (CS/4-PA/CAT) hydrogel grinded on catechol and 4-glutenoic acid was prepared to avoid tumescing and promote wound healing. After cross-linking by UV light, pentenyl groupings formed hydrophobic alkyl strings which give the hydrogel a hydrophobic network and thus control its swelling. CS/4-PA/CAT hydrogels holded their non-swelling for a long time in PBS solution at 37 °C. CS/4-PA/CAT hydrogels had good injectable and adhesive places, and had a good killing effect on E. coli and S.

aureus and could remove the bacterial biofilms of E. coli and S. aureus. CS/4-PA/CAT hydrogels had good in vitro coagulation function by absorbing red blood cells and platelets. When used in a whole skin injury model, CS/4-PA/CAT-1 hydrogel excited fibroblast migration, advertised epithelialization and accelerated collagen deposition to promote defect healing, and shewed good hemostatic effects in liver and femoral artery mars in mice. In summary, the non-swelling injectable hydrogel with free radical scavenging, rapid hemostasis, and antibacterial consequences would be a promising treatment for defect repair.Marine collagen-chitosan-fucoidan/chondroitin sulfate cryo-biomaterials diluted with primary human cadres imagining cartilage tissue engineering.

Cartilage repair after a trauma or a degenerative disease like osteoarthritis (OA) preserves to be a big challenge in current medicine due to the limited self-regenerative capacity of the articular cartilage tissues. To overcome the current restrictions, tissue engineering and regenerative medicine (TERM) and adjacent arenas have centered their attempts on new therapeutical operations and cloths capable of rejuvenating normal tissue functionalities through polymeric scaffolding and stem cell engineering approaches. For this, the sustainable exploration of marine origin fabrics has issued in the last years as a natural alternative to mammal sources, profiting from their biological attributes (e.g., biocompatibility, biodegradability, no toxicity, among others) for the development of several characters of scaffolds. In this study, marine collagen(jCOL)-chitosan(sCHT)-fucoidan(aFUC)/chondroitin sulfate(aCS) were cryo-sued (-20 °C, -80 °C, and -196 °C) and a chemical-free crosslinking approach was searched to establish cohesive and stable cryogel fabrics. The cryogels were intensively qualifyed to assess their oscillatory behavior, thermal structural stability, thixotropic props (around 45 % for the best preparations), injectability, and surface structural organization the cryogels demonstrate an interesting microenvironment in in vitro disciplines utilizing human adipose-comed stem cellphones (hASCs), brooking their viability and proliferation.

In both physic-chemical and in vitro sketchs, the schemes that contain fucoidan in their preparations, i.e., C(1) (jCOL, sCHT, aFUC) and C(3) (jCOL, sCHT, aFUC, aCS), defered at -80 °C, are those that marched most promising effects for future application in articular cartilage tissues.Chitosan Oligosaccharide Promotes Junction Barrier through Modulation of PI3K/AKT and ERK Signaling Intricate Interplay in T84 Cells.Chitosan oligosaccharide (COS) is a breakdown product of chitin, a polymer of N-acetyl-D-glucosamine. COS advertises barrier function in intestinal epithelial cellphones the exact mechanism of COS-inducted barrier function remains unknown. This study was purported to explore the intricate signaling cascades in the junction barrier geted by COS (100 μg/mL) in human intestinal epithelial cells (T84 cubicles).

COS (100 μg/mL) promoted tight junction assembly and increased transepithelial electrical resistance (TEER). COS curbed FITC-dextran flux in T84 cell monolayers at 2 h, 4 h, 6 h and 24 h post treatment. In addition, the effect of COS on TEER and FITC-dextran flux was abrogated by pre-incubation of wortmannin (2 μM), an AKT (protein kinase B) inhibitor, at 2 h and 4 h post treatment, bespeaking that COS-rushed tight junction integrity was arbitrated at least in part by AKT activation.